2IDS
Structure of M98A mutant of amicyanin, Cu(I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.210, 55.100, 26.900 |
| Unit cell angles | 90.00, 95.41, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.000 |
| R-factor | 0.1284 |
| R-free | 0.16950 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1aac |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.069 | 0.297 |
| Number of reflections | 71592 | |
| <I/σ(I)> | 16 | 2.7 |
| Completeness [%] | 92.9 | 74.3 |
| Redundancy | 4.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 293 | 3.0 M phosphate, pH 5.5, EVAPORATION, temperature 293K |
