2IDQ
Structure of M98A mutant of amicyanin, Cu(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.570, 56.160, 26.970 |
| Unit cell angles | 90.00, 96.63, 90.00 |
Refinement procedure
| Resolution | 30.000 - 0.900 |
| R-factor | 0.1078 |
| R-free | 0.13010 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1aac |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.910 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.086 | 0.279 |
| Number of reflections | 52731 | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 84.3 | 37.5 |
| Redundancy | 6.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 293 | 10 mg/ml amicyanin in 3.0 M phosphate, pH 5.5, EVAPORATION, temperature 293K |
