2IDM
2.00 A Structure of T87I/Y106W Phosphono-CheY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-10 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.091, 50.544, 52.956 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
Rwork | 0.233 |
R-free | 0.32800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c4w |
RMSD bond length | 0.015 |
RMSD bond angle | 1.381 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 99.000 | 99.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.073 | 0.050 | 0.443 |
Number of reflections | 6822 | ||
Completeness [%] | 88.1 | 73.1 | 62.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.88 | 298 | 20% PEG 8000, 0.2M ammonium acetate, 0.1 M MES-NaOH, pH 5.88, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |