2IDL
Crystal Structure of Conserved Protein of Unknown Function from Streptococcus pneumoniae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794,0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 52.665, 62.359, 63.415 |
| Unit cell angles | 90.00, 100.92, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.171 |
| Rwork | 0.168 |
| R-free | 0.22637 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.384 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.057 | 0.250 |
| Number of reflections | 20526 | |
| <I/σ(I)> | 21.3 | 3.6 |
| Completeness [%] | 91.5 | |
| Redundancy | 4.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 291 | 1.2M NaH2PO4 /0.8M K2HPO4 Caps 10.5 0.2M Li2So2 , VAPOR DIFFUSION, SITTING DROP, temperature 291K |
