2IDH

Crystal Structure of human FE65 WW domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-29
Detector	ADSC QUANTUM 210
Wavelength(s)	0.975
Spacegroup name	P 63 2 2
Unit cell lengths	75.610, 75.610, 226.489
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.290 - 2.280
R-factor	0.21976
Rwork	0.217
R-free	0.28185
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.925
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.190
Rmerge	0.045
Number of reflections	20584
<I/σ(I)>	43.3
Completeness [%]	99.4
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2.2M Ammonium Sulfate, 0.1M hepes 7.5, 2% PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2.2M Ammonium Sulfate, 0.1M hepes 7.5, 2% PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 293K