2ID3
Crystal structure of transcriptional regulator SCO5951 from Streptomyces coelicolor A3(2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-09 |
Detector | SBC-3 |
Wavelength(s) | 0.97904 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.069, 85.617, 126.341 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.16607 |
Rwork | 0.164 |
R-free | 0.19876 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.506 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.079 | 0.432 |
Number of reflections | 48644 | |
<I/σ(I)> | 31.5 | 3.8 |
Completeness [%] | 99.6 | 96.9 |
Redundancy | 7.7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.2 | 293 | 0.1M Tris7.2, 0.2M CaCl2, 30%PEG400, VAPOR DIFFUSION, temperature 293K |