2ID3
Crystal structure of transcriptional regulator SCO5951 from Streptomyces coelicolor A3(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-09 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.069, 85.617, 126.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.16607 |
| Rwork | 0.164 |
| R-free | 0.19876 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.506 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.079 | 0.432 |
| Number of reflections | 48644 | |
| <I/σ(I)> | 31.5 | 3.8 |
| Completeness [%] | 99.6 | 96.9 |
| Redundancy | 7.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.2 | 293 | 0.1M Tris7.2, 0.2M CaCl2, 30%PEG400, VAPOR DIFFUSION, temperature 293K |
