2ID1
X-Ray Crystal Structure of Protein CV0518 from Chromobacterium violaceum, Northeast Structural Genomics Consortium Target CvR5.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97899 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.188, 76.099, 74.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.680 - 3.000 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.26900 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.680 | 3.150 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.078 | 0.374 |
Number of reflections | 9635 | |
<I/σ(I)> | 24.5 | 5.1 |
Completeness [%] | 99.1 | 100 |
Redundancy | 8.6 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20mM Tris, 16% PEG3350, 125mM ammonium iodide, 8% 1,3-butanediol, and 5 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |