2ID1
X-Ray Crystal Structure of Protein CV0518 from Chromobacterium violaceum, Northeast Structural Genomics Consortium Target CvR5.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.188, 76.099, 74.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.680 - 3.000 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.26900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.680 | 3.150 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.078 | 0.374 |
| Number of reflections | 9635 | |
| <I/σ(I)> | 24.5 | 5.1 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 8.6 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20mM Tris, 16% PEG3350, 125mM ammonium iodide, 8% 1,3-butanediol, and 5 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
