2ICY
Crystal Structure of a Putative UDP-glucose Pyrophosphorylase from Arabidopsis Thaliana with Bound UDP-glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97911 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 188.008, 59.712, 89.762 |
Unit cell angles | 90.00, 100.32, 90.00 |
Refinement procedure
Resolution | 38.250 - 1.640 |
Rwork | 0.183 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z90 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.250 | 50.000 | 1.680 |
High resolution limit [Å] | 1.640 | 4.040 | 1.640 |
Rmerge | 0.042 | 0.032 | 0.295 |
Number of reflections | 118994 | ||
<I/σ(I)> | 20.7 | 3.894 | |
Completeness [%] | 98.8 | 99 | 83.1 |
Redundancy | 4.3 | 4.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapor diffusion, hanging drop with micro-seeding | 273 | Protein solution (10 mg/ml protein, 0.050 M sodium chloride, 0.0003 M TCEP, 0.005 M Tris PH 8.0) mixed in a 1:1 ratio with Well solution ( 28 % PEG 2K, 5% DMSO, 0.10 M MES/Acetate pH 5.5), crystals soaked in well solution supplemented with 0.002 M UDP-glucose, vapor diffusion, hanging drop with micro-seeding, temperature 273K |