2ICQ
urate oxidase under 2.0 MPa pressure of nitrous oxide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 2002-12-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.964 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 80.189, 96.034, 105.212 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.770 - 1.750 |
R-factor | 0.16642 |
Rwork | 0.165 |
R-free | 0.18779 |
Structure solution method | RIGID BODY |
Starting model (for MR) | 2iba |
RMSD bond length | 0.012 |
RMSD bond angle | 1.476 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.055 | 0.346 |
Number of reflections | 41239 | |
<I/σ(I)> | 13.3 | |
Completeness [%] | 99.9 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 298 | urate oxidase 8.5mg/ml, sodium carbonate 10mM, 8-azaxanthin 0.2mg/ml, PEG 8000 7% , pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |