2ICP
Crystal structure of the bacterial antitoxin HigA from Escherichia coli at pH 4.0. Northeast Structural Genomics Consortium TARGET ER390.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.127, 23.526, 41.593 |
| Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.880 |
| Rwork | 0.184 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO (1.2) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.000 | 60.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 5.100 | 1.880 |
| Rmerge | 0.049 | 0.030 | 0.174 |
| Number of reflections | 11592 | ||
| <I/σ(I)> | 18.4 | ||
| Completeness [%] | 90.8 | 97 | 55.1 |
| Redundancy | 2.5 | 2.8 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 200 mM MgCl2, 20% PEG 20K, 100 mM Na3Citrate pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
