2IC7
Crystal Structure of Maltose Transacetylase from Geobacillus kaustophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.479, 104.478, 65.579 |
| Unit cell angles | 90.00, 101.85, 90.00 |
Refinement procedure
| Resolution | 40.520 - 1.780 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ocx |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.467 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 61137 | |
| <I/σ(I)> | 42.95 | 9.27 |
| Completeness [%] | 98.2 | 87.5 |
| Redundancy | 6.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 1 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (10 MG/ML) AND RESERVOIR SOLUTION CONTAINING 3.5M Sodium Formate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.00 |
