2IC6

The Coiled-coil Domain (residues 1-75) Structure of the Sin Nombre Virus Nucleocapsid Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9002
Spacegroup name	P 21 21 21
Unit cell lengths	28.116, 49.521, 100.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.150
R-factor	0.156
Rwork	0.154
R-free	0.17700
Structure solution method	MAD
RMSD bond length	0.021
RMSD bond angle	1.884
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.10)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.170
High resolution limit [Å]	1.150	1.150
Rmerge	0.051	0.238
Number of reflections	50773
<I/σ(I)>	27.4	6.6
Completeness [%]	99.9	99.8
Redundancy	5.8	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.2 M ammonium acetate, 0.1 M sodium acetate, 30% polyethylene glycol (PEG) 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.2 M ammonium acetate, 0.1 M sodium acetate, 30% polyethylene glycol (PEG) 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.2 M ammonium acetate, 0.1 M sodium acetate, 30% polyethylene glycol (PEG) 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K