2IBX
Influenza virus (VN1194) H5 HA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-02 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.678, 100.563, 160.130 |
| Unit cell angles | 90.00, 111.39, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.244 |
| Rwork | 0.241 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jsm |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.665 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.097 | 0.496 |
| Number of reflections | 48951 | |
| <I/σ(I)> | 8.7 | 1.8 |
| Completeness [%] | 76.9 | 80.2 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 15/4 EO/OH, Ammonium sulfate, Bis-tris, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
