2IBJ
Structure of House Fly Cytochrome B5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.201, 40.222, 70.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| Rwork | 0.163 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ljo |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.051 | 0.191 |
| Number of reflections | 14040 | |
| <I/σ(I)> | 17.1 | 8.3 |
| Completeness [%] | 98.2 | 93.3 |
| Redundancy | 6.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 278 | 30% PEG8000, 0.1M Hepes, 0.1M Mg acetate, with microseeding, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
