2IBA
Urate oxidase from Aspergillus flavus complexed with its inhibitor 8-azaxanthine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2005-11-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.972 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 80.492, 96.041, 105.343 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.870 - 1.500 |
R-factor | 0.177 |
Rwork | 0.176 |
R-free | 0.19700 |
Structure solution method | RIGID BODY |
Starting model (for MR) | 1r51 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.408 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.056 | 0.271 |
Number of reflections | 59653 | |
<I/σ(I)> | 10.8 | |
Completeness [%] | 91.1 | 87.9 |
Redundancy | 5.4 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 298 | 10MG/ML URATE OXIDASE, 8-AZAXANTHINE 0.2MG/ML, TRIS 20mM, PEG 8000 7%, NACL 200mM, batch, TEMPERATURE 298K, PH 8.5 |