2IB6
Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone cnidopus japonicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-28 |
| Detector | Bruker Platinum 135 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.079, 126.926, 100.084 |
| Unit cell angles | 90.00, 101.97, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ib5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.644 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | |
| Number of reflections | 121926 | |
| <I/σ(I)> | 39.65 | 4.1 |
| Completeness [%] | 100.0 | 88.5 |
| Redundancy | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.2M NaH2PO4, 20mM Tris-HCl, 150mM NaCl, 20% PEG3350, 20% glycerol, 2mM TCEP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
