2IB5
Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone cnidopus japonicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-09 |
Detector | SBC-2 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.860, 126.850, 100.510 |
Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.22500 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.673 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.077 | |
Number of reflections | 335346 | |
<I/σ(I)> | 12 | 5.1 |
Completeness [%] | 99.3 | 92 |
Redundancy | 52.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.2M NaH2PO4, 20mM Tris-HCl, 150mM NaCl, 20% PEG3350, 20% glycerol, 2mM TCEP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |