2IB0
Crystal structure of a conserved hypothetical protein, rv2844, from Mycobacterium tuberculosis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795,0.9800,0.96 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 98.029, 98.029, 91.337 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.850 - 2.000 |
R-factor | 0.204 |
Rwork | 0.202 |
R-free | 0.23500 |
Structure solution method | MAD |
Starting model (for MR) | None |
RMSD bond length | 0.018 |
RMSD bond angle | 1.575 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.054 | 0.345 |
Number of reflections | 26492 | |
<I/σ(I)> | 13.2 | 2 |
Completeness [%] | 85.8 | 38.7 |
Redundancy | 6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | LiSO4 0.2M Cacodylate pH7 0.1M PEG400 32%, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | LiSO4 0.2M Cacodylate pH7 0.1M PEG400 32%, VAPOR DIFFUSION, SITTING DROP, temperature 298K |