2IAO

Crystal structure of squid ganglion DFPase E37Q mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	2000-01-26
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	42.901, 81.696, 86.246
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.000 - 2.000
Rwork	0.190
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e1a
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	90.000
High resolution limit [Å]	2.000
Number of reflections	40968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	12% PEG 6000, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K