2IAL
Structural basis for recognition of mutant self by a tumor-specific, MHC class II-restricted TCR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 170.044, 65.663, 84.817 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.310 - 1.920 |
| R-factor | 0.21032 |
| Rwork | 0.208 |
| R-free | 0.26453 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fyt |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.869 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.108 | 0.491 |
| Number of reflections | 71032 | |
| <I/σ(I)> | 13.1 | 1.5 |
| Completeness [%] | 78.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 10% PEG-2000 MME, 0.1M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
