2IAI
Crystal structure of SCO3833, a member of the TetR transcriptional regulator family from Streptomyces coelicolor A3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2006-06-09 |
Detector | SBC-3 |
Wavelength(s) | 0.97940 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.308, 61.308, 113.865 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 53.070 - 1.650 |
R-factor | 0.17042 |
Rwork | 0.169 |
R-free | 0.20820 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.654 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.070 | 1.693 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.062 | 0.388 |
Number of reflections | 30460 | |
<I/σ(I)> | 9.2 | 3.7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 10.9 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | Crystallized from mother liquor containing 0.1 M Tris-HCl pH=8.5 and 7%(w/v) PEG 8000, VAPOR DIFFUSION, temperature 293K |