2IA7

Crystal structure of putative tail lysozyme (NP_952040.1) from GEOBACTER SULFURREDUCENS at 1.44 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.94926,0.97925,0.97939
Spacegroup name	P 21 21 21
Unit cell lengths	40.270, 43.332, 64.636
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.315 - 1.440
R-factor	0.169
Rwork	0.168
R-free	0.20300
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.544
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.315	43.300	1.490
High resolution limit [Å]	1.440	3.100	1.440
Rmerge	0.049	0.035	0.439
Number of reflections	19105
<I/σ(I)>	13.38	28.7	1.8
Completeness [%]	90.4	97.8	48.9
Redundancy	3.92	2.35	1.92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP, NANODROP	7.1	277	0.2M LiNO3, 20.0% PEG 3350, pH 7.1, VAPOR DIFFUSION,SITTING DROP, NANODROP, temperature 277K