2I9W

Crystal structure of a sec-c motif containing protein (psyc_2064) from psychrobacter arcticus at 1.75 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.94926,0.97939,0.97925
Spacegroup name	P 21 21 21
Unit cell lengths	41.490, 54.690, 83.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.501 - 1.750
R-factor	0.19392
Rwork	0.192
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.522
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.501		1.810
High resolution limit [Å]	1.750	2.990	1.750
Rmerge	0.056	0.043	0.361
Number of reflections	19754
<I/σ(I)>	9.19	17.6	2.2
Completeness [%]	93.9	98.3	83.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	8.5	277	0.2M MgCl2, 20.0% PEG-8000, 0.1M TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K