2I9U
Crystal Structure of Guanine Deaminase from C. acetobutylicum with bound guanine in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979, 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.385, 88.994, 139.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.500 - 2.050 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.22400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.058 | 0.340 |
| Number of reflections | 64934 | |
| <I/σ(I)> | 17 | 3 |
| Completeness [%] | 93.2 | 53.5 |
| Redundancy | 4.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 10% PEG 3350, 0.1 M Succinic Acid, 10% PEG 400, 0.1 M CaCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
