2I9L

Structure of Fab 7D11 from a neutralizing antibody against the poxvirus L1 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-14
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
Unit cell lengths	218.770, 85.561, 211.843
Unit cell angles	90.00, 119.60, 90.00

Refinement procedure

Resolution	50.000 - 3.100
R-factor	0.24
Rwork	0.240
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ypy 1ymh
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.124	0.464
Number of reflections	61289
<I/σ(I)>	7
Completeness [%]	98.6	99.6
Redundancy	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	298	10% PEG 10000, 0.1M Tris, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.00