2I9E
Structure of Triosephosphate Isomerase of Tenebrio molitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.060, 145.080, 94.280 |
| Unit cell angles | 90.00, 95.61, 90.00 |
Refinement procedure
| Resolution | 29.330 - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.23930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i45 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.250 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.330 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.000 | 0.000 |
| Number of reflections | 53660 | |
| <I/σ(I)> | 11.25 | 2.81 |
| Completeness [%] | 80.4 | 72.4 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | MES, PEG 2000 MME, TRIS, TCEP, EDTA, NaCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
