2I9C
Crystal Structure of the Protein RPA1889 from Rhodopseudomonas palustris CGA009
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-09 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 78.022, 78.022, 38.979 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.790 - 2.000 |
| R-factor | 0.17603 |
| Rwork | 0.175 |
| R-free | 0.20189 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.605 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.570 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.299 |
| Number of reflections | 9449 | |
| <I/σ(I)> | 6.83 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 5% Tascimate, 10% PEG5000 MME, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
