2I8U
GDP-mannose mannosyl hydrolase-calcium-GDP product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 180 mm plate |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.588, 81.151, 97.982 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.840 - 1.400 |
| R-factor | 0.142 |
| Rwork | 0.140 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.653 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.041 | 0.100 |
| Number of reflections | 77746 | |
| <I/σ(I)> | 18.5 | |
| Completeness [%] | 98.7 | 96.4 |
| Redundancy | 4.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 281 | 10-20% PEG 4000 200 mM sodium acetate 100 mM HEPES, pH 8.0 harvested 72 hours after growth, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
