2I8E
Structure of SSO1404, a predicted DNA repair-associated protein from Sulfolobus solfataricus P2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2006-06-09 |
Detector | SBC-3 |
Wavelength(s) | 0.97940 |
Spacegroup name | P 62 |
Unit cell lengths | 64.277, 64.277, 39.529 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.940 - 1.590 |
R-factor | 0.18972 |
Rwork | 0.188 |
R-free | 0.22708 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.692 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.650 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.052 | 0.309 |
Number of reflections | 12619 | |
<I/σ(I)> | 63.9 | 8.2 |
Completeness [%] | 99.5 | 96.4 |
Redundancy | 11.7 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Crystallized in 0.2M Na iodide, 20% PEG3350, 2% isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |