2I8D

Crystal structure of an uncharacterized conserved protein of COG5646 (ZP_00384875.1) from Lactobacillus casei ATCC 334 at 1.69 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.94926,0.97925
Spacegroup name	P 21 21 21
Unit cell lengths	47.946, 69.276, 90.872
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.775 - 1.690
R-factor	0.169
Rwork	0.168
R-free	0.19700
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.365
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.775	29.780	1.730
High resolution limit [Å]	1.600	7.560	1.690
Rmerge	0.081	0.069	0.662
Total number of observations		1369	9186
Number of reflections	34612
<I/σ(I)>	4.7	5.8	1.1
Completeness [%]	99.9	95.6	100
Redundancy	3.6	3.2	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	4	277	1.0M LiCl, 20.0% PEG-6000, 0.1M Citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP,