2I8D
Crystal structure of an uncharacterized conserved protein of COG5646 (ZP_00384875.1) from Lactobacillus casei ATCC 334 at 1.69 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.94926,0.97925 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.946, 69.276, 90.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.775 - 1.690 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19700 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.365 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.775 | 29.780 | 1.730 |
| High resolution limit [Å] | 1.600 | 7.560 | 1.690 |
| Rmerge | 0.081 | 0.069 | 0.662 |
| Total number of observations | 1369 | 9186 | |
| Number of reflections | 34612 | ||
| <I/σ(I)> | 4.7 | 5.8 | 1.1 |
| Completeness [%] | 99.9 | 95.6 | 100 |
| Redundancy | 3.6 | 3.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 4 | 277 | 1.0M LiCl, 20.0% PEG-6000, 0.1M Citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, |
