2I8A

Salmonella typhimurium liganded by phosphate ion at 1.64A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-15
Detector	MAR CCD 165 mm
Wavelength(s)	0.8
Spacegroup name	P 21 21 21
Unit cell lengths	89.600, 125.030, 134.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.000 - 1.640
R-factor	0.19883
Rwork	0.197
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.233
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	1.682
High resolution limit [Å]	1.640	1.640
Number of reflections	241458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	VAPOR DIFFUSION, HANGING DROP, temperature 297K