2I7X
Structure of Yeast CPSF-100 (Ydh1p)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 75.631, 122.801, 126.862 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.180 - 2.500 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.29000 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB (THEN SOLVE/RESOLVE) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.087 | 0.408 |
Number of reflections | 20806 | |
<I/σ(I)> | 24.4674 | 5.644 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.3 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 20% PEG 3350, 0.2M ammonium citrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |