2I7R
conserved domain protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-27 |
Detector | SBC-3 |
Wavelength(s) | 0.979321 |
Spacegroup name | P 65 |
Unit cell lengths | 97.624, 97.624, 55.151 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.560 - 2.200 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.25700 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.691 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.096 | 0.459 |
Number of reflections | 15324 | |
<I/σ(I)> | 8.5 | |
Completeness [%] | 99.4 | 99.2 |
Redundancy | 12.7 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.100 M sodium citrate 2.00 M sodium chloride, pH 4.50, VAPOR DIFFUSION, SITTING DROP, temperature 289K |