2I7G
Crystal Structure of Monooxygenase from Agrobacterium tumefaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-22 |
Detector | SBC-3 |
Wavelength(s) | 0.97908 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 99.515, 139.974, 54.049 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.370 - 1.730 |
R-factor | 0.17292 |
Rwork | 0.170 |
R-free | 0.20199 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.405 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.370 | 1.790 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.086 | 0.503 |
Number of reflections | 78621 | |
<I/σ(I)> | 8.3 | 2 |
Completeness [%] | 98.4 | 87.5 |
Redundancy | 10.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.1M Bis-tris pH 5.5, 0.2M Ammonium sulfate, 25% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |