2I79
The crystal structure of the acetyltransferase of GNAT family from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-23 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 133.131, 174.792, 56.573 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.700 - 2.100 |
| R-factor | 0.21045 |
| Rwork | 0.208 |
| R-free | 0.25133 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.593 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.083 | |
| Number of reflections | 68790 | |
| <I/σ(I)> | 26.3 | 2 |
| Completeness [%] | 93.5 | 62.52 |
| Redundancy | 8.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 35%MPD, MES pH6.0, 0.2M LiSO4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
