2I71
Crystal structure of a Conserved Protein of Unknown Function from Sulfolobus solfataricus P2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-21 |
Detector | SBC-3 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.849, 84.918, 108.513 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.920 - 1.700 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.22744 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.533 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.920 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.086 | 0.492 |
Number of reflections | 80005 | |
<I/σ(I)> | 28.98 | 3.7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 283 | 25% PEG3350, 0.2M MgCl2, 0.1M BisTris, 2% Tacsimate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K |