2I71
Crystal structure of a Conserved Protein of Unknown Function from Sulfolobus solfataricus P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-21 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.849, 84.918, 108.513 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.920 - 1.700 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22744 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.533 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.920 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.086 | 0.492 |
| Number of reflections | 80005 | |
| <I/σ(I)> | 28.98 | 3.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 283 | 25% PEG3350, 0.2M MgCl2, 0.1M BisTris, 2% Tacsimate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
