2I6V
PDZ domain of EpsC from Vibrio cholerae, residues 219-305
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-05-03 |
Detector | MAC Science DIP-2030B |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.182, 44.884, 51.155 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.990 - 1.630 |
Rwork | 0.170 |
R-free | 0.20300 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.108 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.690 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.044 | 0.187 |
Number of reflections | 10558 | |
<I/σ(I)> | 57.6 | 16.7 |
Completeness [%] | 99.4 | 97.3 |
Redundancy | 5.4 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.8 | 294 | 1.9M ammonium sulfate, 0.2M Li sulfate, 0.1M Tris pH 7.8, vapor diffusion, temperature 294K |
1 | VAPOR DIFFUSION | 7.8 | 294 | 1.9M ammonium sulfate, 0.2M Li sulfate, 0.1M Tris pH 7.8, vapor diffusion, temperature 294K |