2I6T

Orthorhombic Structure of the LDH domain of Human Ubiquitin-conjugating Enzyme E2-like Isoform A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-07-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	53.088, 98.912, 126.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.550 - 2.100
R-factor	0.18267
Rwork	0.180
R-free	0.23004
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i10
RMSD bond length	0.013
RMSD bond angle	1.412
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.550	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	39716
<I/σ(I)>	12.6	2.88
Completeness [%]	99.9	100
Redundancy	7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	26% PEG3350, 0.2 M ammonium sulfate, 0.1 M sodium cacodylate, pH 5.2 in protein buffer comprising of 20 mM Tris-HCl, pH 8.0, 500 mM NaCl, 5% glycerol, 10 mM dithiothreitol, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K