2I6R
Crystal structure of E. coli HypE, a hydrogenase maturation protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.10000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 254.757, 71.776, 113.034 |
| Unit cell angles | 90.00, 115.12, 90.00 |
Refinement procedure
| Resolution | 43.730 - 2.510 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.328 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.071 | 0.036 | 0.303 |
| Number of reflections | 63144 | ||
| <I/σ(I)> | 12.4 | ||
| Completeness [%] | 99.3 | 99.3 | 95.5 |
| Redundancy | 3.7 | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | 1.28 M NaK phosphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
