2I6H
Structure of Protein of Unknown Function ATU0120 from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.490, 101.193, 103.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.000 - 1.750 |
| R-factor | 0.1676 |
| Rwork | 0.166 |
| R-free | 0.19920 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.413 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.900 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.127 | 0.850 |
| Number of reflections | 42671 | |
| <I/σ(I)> | 25.1 | 1.95 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 13.7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M calcium chloride, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
