2I6F
Receiver domain from Myxococcus xanthus social motility protein FrzS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.116 |
| Spacegroup name | I 4 |
| Unit cell lengths | 142.479, 142.479, 37.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.22167 |
| Rwork | 0.220 |
| R-free | 0.25369 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gkg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.826 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 30067 | |
| <I/σ(I)> | 6.8 | 1.6 |
| Completeness [%] | 98.5 | 98.7 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | PEG 3350, NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
