2I6E
Crystal structure of protein DR0370 from Deinococcus radiodurans, Pfam DUF178
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9802 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.310, 139.370, 153.615 |
| Unit cell angles | 90.00, 92.79, 90.00 |
Refinement procedure
| Resolution | 48.030 - 2.500 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.23500 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.030 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.095 | 0.409 |
| Number of reflections | 107765 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 98.9 | 91 |
| Redundancy | 6.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 25% PEG 3350 0.2 M Ammonium Sulfate 0.1 M B-s-Tris pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
