2I6D
The structure of a putative RNA methyltransferase of the TrmH family from Porphyromonas gingivalis.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97951, 0.97962 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.921, 107.119, 120.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.100 - 1.850 |
| R-factor | 0.19126 |
| Rwork | 0.189 |
| R-free | 0.23470 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.415 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 23206 | |
| <I/σ(I)> | 11.4 | 2.35 |
| Completeness [%] | 98.3 | 89.2 |
| Redundancy | 3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 0.8M NaH2PO4, 1.2M K2HPO4, 0.1M Acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
