2I5Z
The crystal structure of OspA mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-17 |
Detector | SBC |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.783, 53.840, 65.495 |
Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.200 |
R-factor | 0.14945 |
Rwork | 0.148 |
R-free | 0.17599 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g8c |
RMSD bond length | 0.011 |
RMSD bond angle | 1.397 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.071 | 0.265 |
Number of reflections | 70928 | |
<I/σ(I)> | 15.59 | 2.43 |
Completeness [%] | 95.2 | 91.6 |
Redundancy | 2.3 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 34% PEG400, 0.1M Tris-HCl, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |