2I5Q

Crystal structure of Apo L-rhamnonate dehydratase from Escherichia Coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97911
Spacegroup name	I 4
Unit cell lengths	124.825, 124.825, 103.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.890 - 2.100
R-factor	0.229
Rwork	0.229
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rvk
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.100
Number of reflections	44477
Completeness [%]	95.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	1.2 M ammonium sulfate, 0.1M Tris-HCl, 0.2 M Lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K