2I5N
1.96 A X-ray structure of photosynthetic reaction center from Rhodopseudomonas viridis:Crystals grown by microfluidic technique
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Detector technology | CCD |
Collection date | 2006-07-17 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 220.400, 220.400, 113.009 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.960 |
R-factor | 0.173 |
Rwork | 0.172 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dxr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.329 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.082 | 0.480 |
Number of reflections | 189189 | |
<I/σ(I)> | 17.5 | 2.3 |
Completeness [%] | 94.5 | 67.4 |
Redundancy | 5.9 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | THE CRYSTALS WERE GROWN BY MICROFLUIDIC TECHNIQUE USING CRYSTALLIZATION BUFFER: 1.6 - 1.8 M (NH4)2SO4 IN TRIS PH 7.8. THE ADDITIVES WERE HEPTANETRIOL (3%, W/V) AND 2% TRIETHYL AMONIUM PHOSPHATE. THE DETERGENT WAS LAURYL DIMETHYLAMINE-N-OXIDE (LDA). THE PROTEIN COMPLEX WAS IN SODIUM PHOSPHATE BUFFER PH 6.0 AND 0.08% LDA. |