2I5G
Crystal strcuture of amidohydrolase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.644, 150.615, 68.828 |
| Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.600 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.119 | 0.272 |
| Number of reflections | 43327 | |
| Completeness [%] | 99.2 | 99.5 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Cacodylate, 22.5% PEG400, 0.2M LiSO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
