2I56

Crystal structure of L-Rhamnose Isomerase from Pseudomonas stutzeri with L-Rhamnose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	74.616, 104.757, 114.292
Unit cell angles	90.00, 107.96, 90.00

Refinement procedure

Resolution	33.250 - 1.970
R-factor	0.17
Rwork	0.170
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SeMet-substituted model of L-rhamnose isomerase
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.040
High resolution limit [Å]	1.970	1.970
Rmerge	0.104	0.242
Number of reflections	117301
<I/σ(I)>	9.5
Completeness [%]	99.5	95.1
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	298	7-8% (w/v) polyethylene glycol 20000, 50mM MES, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K