2I55
Complex of glucose-1,6-bisphosphate with phosphomannomutase from Leishmania mexicana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Collection date | 2005-11-08 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.224, 92.224, 172.824 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.700 - 2.900 |
| Rwork | 0.192 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i54 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.371 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.065 | 0.038 | 0.311 |
| Number of reflections | 17996 | ||
| <I/σ(I)> | 17.3 | ||
| Completeness [%] | 90.6 | 89.4 | 84.5 |
| Redundancy | 3.6 | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 14% (w/v) PEG 3350, 10% PEG 400, 0.1M citrate, 50% PEG 3550, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
