2I53
Crystal structure of Cyclin K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.766, 69.186, 50.504 |
| Unit cell angles | 90.00, 93.30, 90.00 |
Refinement procedure
| Resolution | 23.690 - 1.500 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.982 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.071 | 0.217 |
| Number of reflections | 46197 | |
| <I/σ(I)> | 32.28 | 6.5 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 10.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Ammonium sulfate, PEG 400, HEPES, PH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
